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KMID : 0357319960310020165
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Journal of the Korean Society for Microbiology
1996 Volume.31 No. 2 p.165 ~ p.174
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Study on the Development of a Rapid Detection Method for Pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis by Polymerase Chain Reaction
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오호정
김창민/성원근/민홍기
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Abstract
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We developed a polymarase chain reaction (PCR) method to detect and identify pathogenic Yersinia enterocolitica and Y. pseudotuberculosis. Sixty-four Y. enterocolitica strains, twenty-sixother Yersinia species and thirteen other
Enterobacteriaccaestrains were collected in Korea and around the world. PCR was performed by using primers against the ail gene from pathogenic Y. enterocolitica and the inv gene from Y. pseudotuberculosis, respectively and simultaneously. The
458
bp
DNA fragment was amplified from 53 pathgenic Y. enterocolitica which contained the ail gene. Any product was not amplified from non-pathogenic Y. enterocolitica, nor from other Yersinia species and thirteen other strains of the
Enterobacteriaceae.
This
suggested that PCR using the ail gene probe have 100% sensitivity and 100% specificity to detect pathogenic Y. enterocolitica. The 684 bp DNA fragment was amplified from 13 Y. pseudotuberculosis which contained the inv gene. Any product was not
amplified from Y. enterocolitica, nor from other Yersinia species and other strains of the Enterobacteriaccae. This result suggested that PCR using the inv gene probe have 100% sensitivity and 100% specificity to detect Y. pseudotuberculosis. The
One
Shot PCR using a mixture of the ail and inv primers made it possible to detect pathogenic Y. enterocolitica and Y. pseudotuberculosis, respectively at the same time. Our results were proved to be a rapid and convenient procedure for routine
detection
and identification of Y. enterocolitica and Y. pseudotuberculosis, respectively and simultaneously.
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